g. Recombination. Plasmid pBABE GFP from Dr. , GFP or luciferase) that is either co-transfected with the test plasmid or transfected into a separate well of your cells. Pmax Gfp Plasmid, supplied by Amaxa, used in various techniques. ZERO BIAS - scores, article reviews, protocol conditions and more. Addgene (RRID: Addgene_34632). They contain two separate transcription units (TU), each consisting of a strong promoter and an efficient polyadenylation signal. To establish stable cell lines constitutively expressing GFP, three GFPs expressed from plasmid pBIEGFP, pSG5GFP, and pRSGFP were introduced into NIH/3T3, BHK-21, Huh-7, and HepG2 cells. A. This chapter describes the use of the GFP-promoter-probe plasmid, pJSK411, which is suitable for the evaluation of differential gene expression in Plasmid Pph Akt Gfp Encoding Ph Akt Gfp, supplied by Addgene inc, used in various techniques. The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. 이 기회에 더 좋은 vector를 구입할까 하는데, 사용하시는것 중에 괜찮은 GFP vector있으면 추천해 주시면 감사  GFP Vector Set (plasmid vectors for molecular cloning); Molecular cloning often benefits from optimizing the vector used for expression; A green fluorescent protein (GFP)-based plasmid system to study post- transcriptional control of gene expression in vivo. - (reply: 1) constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1) This GFP reporter plasmid expresses CD44CR1, a noncoding sequence upstream of the CD44 locus. This vector  A dual marker plasmid comprising the reporter gene sgfp (green fluorescent protein) and the selectable bar gene (Basta tolerance) was constructed by. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene. Using Yeast Homologous. The shRNA expression is suppressed by Tet repressor (TetR) binding to the hybrid H1 promoter containing tetracycline operators (TetO) and RNA interference only takes place in the presence of tetracycline. 2004 Jan 6. RDB08657 pSKY54-SEC12 Plasmid clone to express SEC12 protein tagged with GFP in S. Basic Cloning Vectors; CRISPR Plasmids; Fluorescent Protein Genes & Plasmids; GFP; Gateway ® Cloning Vectors; I. g plasmids, BAC) into a bacterium. GFP and its homologues have a beta-barrel structure. 34. pAV-ALB. ampicillin resistance (ampR) 3. 1998 Mar 12. . Please select your country: Please contact your distributor for pricing. The Cellometer Vision CBA  28 Oct 2012 A plasmid that gives a bacterium the ability of producing sexual pilus for conjugation. The Cas9-D10A nickase plasmid co-expressing GFP uses the CMV promoter for strong transient expression of Cas9-D10A. Utilizing recent improvements in molecular techniques for a time‐sensitive environment, undergraduates were able to create a chimera of a selected gene and green fluorescent protein (GFP) in a bacterial expression plasmid over the course of a single semester in a weekly 3‐h laboratory period. The maps for  Tripartite Split-GFP Association에 기초한 새로운 단백질 - 단백질 상호 작용 센서. 2017년 4월 20일 The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26. In my experiments with HeLa transfected with pCDNA3 beta-eGFP plasmid, the GFP expression remains till 72 hours after transfection. Confers resistance to 100 µg/mL ampicillin. coli bacteria and pGLO, a plasmid modified with three genes. Strong expression of GFP fusion proteins was a … Search term: "cas 9 GFP plasmid" Compare Products: Select up to 4 products. Transformants were selected on a kanamycin-supplemented agar plate and incubated at 37°C, resulting in the loss of the pKD46 cloning vector from the cells. 8(6):343-6. A PEI-poly(ethylene glycol)-folate (PEI-PEG-FOL) conjugate was synthesized as a pSUPER-siGFP plasmid gene carrier. 2009;540: 301-19. Plasmid GFP-C1-PLCdelta-PH from Dr. This recombinant plasmid, created by researchers at Bio-Rad, combines a gene for green fluorescent protein (GFP), cloned from a jellyfish, with control elements copied from a bacterial operon. Quantitative flow cytometry was used to esti- mate plasmid copy number and GFP (S65T) molecules in single cells. 9 kDa) that exhibits bright green fluorescence when  2019년 12월 10일 제한효소를 통해, 벡터 플라스미드의 Multicloning site 확보. edu Plasmid Background: In this lab, you will be using non-pathogenic E. 2017 Feb 15. a green fluorescent protein (GFP) from the bioluminescent jellyfish, Aequorea victoria 2. Comments. Plasmid Sets. Does anyone know if there are We report the construction of a versatile GFP expression plasmid and demonstrate its utility in Neurospora crassa. cerevisiae. they allow for strong and sustained co-expression of two reporter genes: GFP and LacZ. To ligate, an enzyme called T4 ligase is used. 2159. 18 Aug 2020 GFP siRNA and shRNA Plasmids (A. 17 Sep 2015 Plasmid map of pAC-plac-gfp. 1-29. Bioz Stars score: 92/100, based on 5 PubMed citations. Email To Buyer Print this Page Copy Link Ordering. Normally I observe the highest expression in between 36-48 hours Lentiviral vector with ampicillin selection and CMV promoter and Myc and FLAG tags. Another method is to use a GFP that contains a mutation where the fluorescence will change from green to yellow over time, which is referred to as a fluorescent timer. Jeanne Hardy's lab contains the inserts CA-GFP and mLumin and is published in J Biol Chem. William Hahn's lab. This gene is expressed under the control of the P lac promoter. Optimized mTagBFP2. The pGLO plasmid contains the genetic codes for (see Table 2): 1. 예전부터 쓰던 GFP vector가 깨진듯 합니다. 1039/c5ib00232j. Co-delivery of doxorubicin and TRAIL plasmid by modified PAMAM dendrimer in colon cancer cells,  Browse our green, red, yellow, cyan and far red fluorescent protein plasmids. Expression plasmids pVITRO1-GFP/LacZ are developed mainly for in vitro studies. Detection pmaxGFP plasmid, carrying the gene encoding green fluorescent protein (GFP) under the regulation of the cytomegalovirus (CMV) promoter, was supplied by Amaxa (Gaithersburg, MD) as a part of an electroporation kit. BFP2. More than 20 requests For example, GFP can be included in a plasmid expressing other genes to indicate a successful transfection of a gene of interest. This clone was derived from ATCC ® 25922™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. 101(1):16-22. The BioBricks Prefix and Suffix were then prepended and appended to the start of plac and end of gfp specific  16 Jan 2020 (IPTG) on the expression of enhanced green fluorescent protein (eGFP) The variation of specific eGFP production and plasmid copy number  8 Mar 2016 green fluorescent protein (GFP) and the percentage of initial cells expressing GFP were both decreasing with the plasmid size increase. Fusions in Plasmids. ZERO BIAS - scores, article reviews, protocol conditions and more Home » Resources » Plasmid Files » Mammalian Expression Vectors » pCMV6-AC-IRES-GFP pCMV6-AC-IRES-GFP PrecisionShuttle™ mammalian expression vector with a TurboGFP marker, for fusing an ORF to C-terminal c-Myc and FLAG® tags. 1 Feb 2009 We have investigated the impact of plasmids and GFP expression on invasion of cultured epithelial cells by Salmonella enterica Typhimurium  8 Jul 2015 GFP tagging (Green Fluorescent Protein fusion) - This lecture explains about the green fluorescent protein tagging technique and the use of  Reporter gene plasmids with luc, GFP, lacZ. David Feldser's lab contains the insert GFP and is published in Cancer Res. Connie Cepko's lab contains the insert EGFP from Aequorea victoria and is published in Proc Natl Acad Sci U S A. mTagBFP2. 2011 Jul 15;286(28):24977-86. Expression of the GFP-LC3B fusion gene allows to visualize autophagosome formation in real time in live cells. pii: canres. 1/NT-GFP Vector for expressing N-terminally GFP-tagged proteins in mammalian cells. Another type of transfection control is an internal control vector, which measures transfection efficiency. 301-251-6638 Plasmid Protocol . 1158/0008-5472. See full list on embryo. 2011;807:25-46. To visualize nuclei and microtubules, we generated carboxy-terminal fusions of sgfp to Neurospora histone H1 (hH1) and beta-tubulin (Bml). pGLO plasmids contain two genes that are useful: the gene coding for beta-lactamase and the gene coding for the green ¬‚uorescent protein (GFP). Group Size: For 10 lab groupsTime Required: Set Up - 50 minutes / Incubation - overnight / Transformation - 15 minutesKit Includes: Instructions, BactoBeads™ E. 26(1):3-11. doi: 10. To investigate the mechanism by which plasmid carriage decreases invasion, we monitored SPI-1 gene expression using prgH promoter activity as an index of SPI-1 activity. Green fluorescent protein (GFP) is a protein that glows with a bright green fluorescence under ultraviolet light. 1 NT-GFP pcDNA™3. The expression of beta-lactamase gene provides resistance to the antibiotic ampicillin. pGLO Lab Kits utilize Bio-Rad’s pGLO Plasmid, which encodes a green fluorescent protein (GFP), to enable instructors to give students a hands-on introduction to transformation, cloning, protein chromatography, and electrophoresis techniques. No Arabinose = NO GLOW Transformation • Transformation is the process of putting a plasmid into a bacterial cell. RDB08653 pQR316-SEC71TMD-GFP Plasmid clone to express SEC71 transmembrane domain tagged with GFP in S. Amaxa pmax gfp plasmid. The protein consists of 238 amino acid residues that  Access to cell lines and plasmids is only provided to investigators who have This plasmid contains two loxP sites and a neo cassette flanked by FRT sites. Consortium Plasmids; Insect Cell Vectors; Luciferase Vectors; Lucigen Vectors; Mammalian Expression Vectors; pET & Duet Vectors (Novagen) pGEX Vectors (GE Healthcare) Plant Vectors; Qiagen Vectors Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. Compare minicircle DNA and the corresponding plasmid for optimized transfection. During The plasmid is used for 2019-nCoV (SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). Transformation is the process of introducing foreign DNA ( e. Menu. Epub 2011 May 10. Green fluorescent protein (GFP) has become more popular to be used as a living marker for positively transfected clones in many studies. pAAV-GFP Control Plasmid. Plasmid pcDNA3-EGFP from Dr. Despite the effect of plasmid-encoded CmR, the strain containing chromosomally encoded GFP, also carrying a CmR gene, was as invasive as the wild-type. Reactivity Human Conjugate GFP tag Application Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI) Purpose With the Lamin-Chromobody®plasmid you can observe nuclear morphology in real time in your cell line of interest. (No, the plasmid sizes add up to the size of pUC + GFP. In the next step we want to sort and amplify our newly prepared GFP plasmid. Plasmids - pVectOZ · Green Fluorescent Protein (GFP) – pVectOZ-GFP · beta- Galactosidase (Lac Z) – pVectOZ-LACZ · Luciferase (Luc) – pVectOZ-LUC · Secreted  pLL-EF1a-GFP-T2A-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus. These vectors express the Cycle 3 Green Fluorescent Protein (Cycle 3 GFP) which has spectral excitation and emission wavelengths identical to wild-type GFP. shRNA lentivirus vector with H1 promoter and GFP and ampicillin selection. 6 KB) Related Products . pSELECT-Tag features three well-known tags: the green fluorescent protein (GFP) gene, the human influenza hemagglutinin (HA) epitope, the polyhistidine (His) tag, and, a novel secreted luciferase (Lucia). Assuming that the inserted piece of DNA starts at the first position of the triplet codon, then in this stretch of plasmid DNA, where is the best place to insert DNA? Plasmid pRB30-GFP from Dr. GFP was isolated from the jelly fish Aequorea victoria. - DNA연결효소를 통해, 플라스미드와 타겟유전자 서열 연결. BioTechniques 34:74-80 (January 2003). 2016 Apr 18;8(4):542-5. 1039/c8ra06237d. Methods Mol Biol. 2018 Sep 27;8(58):33087-33095. Plasmid pAAV-GFP from Dr. Home » Resources » Plasmid Files » Mammalian Expression Vectors » pcDNA3. Plasmid pYUB1062-GFP from Dr. One of the limitations is the size of GFP: ~240 amino acids or about 28 kDa. The CD19 receptor gene was obtained from OriGene Technologies (Rockville, MD). pSELECT-GFP-LC3 are mammalian expression vector containing the human or murine LC3B gene fused at its 5’ end to the green fluorescent protein (GFP) gene. The plasmid pVSV-ΔG-GFP-2. First, colon cancer  The High-Efficiency gRNA-Cas9-GFP plasmid (linear) Assembly Kit is used for easier and highly efficient cloning of gRNA fragments into Cas9 vectors. Epub 2015 Nov 27. Bio-Rad Explorer pGLO Plasmid and GFP Kits use the pGLO plasmid, which contains the GFP gene, to enable hands-on learning about the central dogma, gene expression and regulation, bacterial transformation, protein separation, and the biomanufacturing process. pSELECT-GFP-LC3 is a mammalian expression vector containing the human LC3B gene fused at its 5’ end to the green fluorescent protein (GFP) gene. Size . PDF (557. The pVL-GFP vector was derived by cloning a PCR fragment encoding the entire ORF of the Aequorea victoria green fluorescent protein into the pVL1393 vector. coli shuttle plasmid  8 Oct 2019 GFP. Expression of the GFP-LC3 fusion gene allows to visualize autophagosome formation in real time in live cells. Using a  2 Aug 2017 In order to test the efficiency of our plasmid-curing system, we constructed three target plasmids by inserting gfp under control of a constitutive  The gWiz plasmids are designed for the highest level of gene expression in a wide range of Notes, GFP expression in mammalian cells and tissues  22 Jul 1997 In this report, we describe experiments using Aequorea victoria green fluorescent protein (GFP) as a reporter in both bulk cultures and individual  All bacterial strains and plasmids used in this work are described in Table 1. Elizabeth Fullam's lab is published in RSC Adv. ZERO BIAS - scores, article reviews, protocol conditions and more https://www. victoria) · Target species: A. ps2906s74. 1007/978-1-59745-558-9_22. Also, the Cas9D10A expression plasmids can be linearized using XbaI for T7-based mRNA production. Bioz Stars score: 88/100, based on 7 PubMed citations. CAN-16-2159. Vector for fusing EGFP to the C-terminus of a partner protein. Reliably label cells with copGFP driven by a moderate EF1α promoter  (That is correct) The plasmids recircularized. Plasmid CAG-GFP from Dr. Not for Use in Therapeutic or Diagnostic Procedures. · Gene Silencers generally consist of pools of three to five target-specific 19-25  This collection of plasmid vectors includes basic cloning and subcloning vectors firefly, NanoLuc® and Renilla luciferases, GFP and dual-reporter coincidence  1 Apr 2020 Although the jellyfish Aequorea Victoria Green fluorescent protein (GFP) was already discovered in the 1960s, it took three more decades until  16 Mar 2011 Scientific Background. Bacterial cells into which  You want to “cut” G-Protein out of this plasmid, and “paste” it in to a GFP Expression plasmid (right) between the mammalian promoter and GFP. pBSU101. Bioz Stars score: 85/100, based on 10 PubMed citations. The GFP gene is ligated into the open plasmid to recreate a circle. The plasmid pVSV-ΔG-GFP encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with GFP. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed from a separate plasmid. Lentiviral vector with ampicillin selection and CMV promoter and C-terminal GFP. 6 Aug 2020 We also applied GFP DNA plasmid, which would produce green fluorescent protein after it was delivered into cells by GQDs. 2 matches found for cas 9 GFP plasmid General description. 2013 Nov 5;74:29. BFP1. For other reading frames, use pEGFP-N2 or pEGFP-N3. bioz. Inducible pLV-RNAi vector system is designed to deliver gene silencing in a tightly regulated fashion in mammalian cell lines. Doug Golenbock's lab contains the insert Enhanced Green Fluorescent Protein. Alternate promoters can be substituted by replacement of CMV using MluI and NheI. The end result is a system that allows for bacterial expression of a eukaryotic gene. Tobias Meyer's lab contains the insert PLCdelta and is published in Curr Biol. ABSTRACT. Fred Gage's lab contains the insert eGFP and is published in J Neurosci. E. pAV • AraC activates expression of GFP when Arabinose is present in the media. The outcome is a closed plasmid containing our GFP gene. G. While the pLL-CMV-GFP-T2A-Puro Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells. GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea victoria. pVITRO1-GFP/LacZ plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic pVL-GFP: Cat. More than 20 requests GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post, so stay tuned! Transient transfection and the elusive "mammalian ORI" For many experiments, it is sufficient for the transfected plasmid to be expressed transiently. This plasmid is available through Addgene . The plasmid pVSV-ΔG-GFP encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in  Apoptosis 2019 ,PubMed ID 31654241 [pCMV6-AC-GFP]. Plasmid LentiCRISPRv2GFP from Dr. Renato Bruni's lab is published in Curr Protoc Protein Sci. GeneArt® Thermo Fisher Scientic®. 2) 박테리아 형질전환 및  31 Oct 2018 Green Fluorescent Protein, or GFP, is widely used to study transfection efficiency and monitor intra-cellular events. Sep 30, 2019 · Ampicillin resistance gene ( bla) encoded on a plasmid. Thermo Scientific pT7CFE1 CGFP HA His is a cloning plasmid optimized to use with the Thermo Scientific 1 Step Human In Vitro Protein Expression System for in vitro translation IVT of tagged fusion proteins pT7CFE1 CGST HA His Vector is available with tandem affinity tags GFP HA and 6xHis at the C terminus to facilitate protein purification and detection pT7CFE1 CGST HA His also has a cleavable If the GFP gene is inserted correctly, there should be no stop codons and a GFP- -gal fusion protein will be made from the mRNA. John T Gray's lab contains the insert GFP and is published in Methods Mol Biol. Remember the genetic code is read as triplet codons. This plasmid is available through Addgene. A GFP siRNA plasmid vector (pSUPER-siGFP), which inhibits the synthesis of GFP, was constructed and used for suppressing GFP expression in folate receptor over-expressing cells (KB cells) in a target-specific manner. AAV9 with CMV promoter-driven expression of GFP. *Please select more than one item to compare. Plasmid pGLO-GFP-1UAG from Dr. Each component plasmid contains the green fluorescent reporter regulated in a different way - in the MCS under the CMV promoter, under an IRES or regulated by your own chosen promoter, or regulated by independent promoters either immediately downstream of the MCS (sharing a polyA with the gene inserted into the MCS) or in a different part of the plasmid. This plasmid is selectable in bacteria and mammalian cells using Zeocin™. GFP reporter systems have also been used to evaluate in vivo gene expression in other organisms including Staphylococcus aureus (6), Listeria monocytogenes (7), and Mycobacterium marinum (8). 6 encodes the antigenomic-sense (or positive-sense) RNA of a replication-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with a multiple cloning site (5'-MluI- XmaI-SmaI-EagI-NheI-3') and that contains an additional transcription unit between the MCS and Lonza provides a plasmid map of pmaxGFP showing the KpnI, NheI, Eco47III, and AgeI restriction sites upstream of max GFP and BglII, XhoI, and SacI downstream. Although nearly as bright as the Vivid Colors™ FPs, you’ll need to use the proper filter set to observe the fully-enhanced brightness. Plasmid pT-CA-GFP-IRES-mLumin from Dr. . GFP encoding plasmid DNA (25 µg) Developed and manufactured by Altogen Biosystems; Kit includes 25 µg of GFP plasmid DNA; DNA transfection control for fluorescent microscopy; Transfection control for chemical transfection and electroporation; Validated for use in human cell lines; Can be used for plasmid DNA / GFP siRNA co-transfection A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo Methods Mol Biol . Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein published in 2005, derived from Aequorea victoria. pRed_Cas9_recA_∆poxb300 pSELECT-Tag is a family of expression plasmids designed to generate tagged proteins in order to facilitate their detection and/or purification. asu. 2006 Jan 4. The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research. This system amplifies the intensity of the fluorescent signal and enables single molecule tracking within living cells without affecting protein function, thereby creating a single-molecule reporter of intracellular processes. 2016. EGFP. The gfp-oriTpIP501 cassette was inserted into the E. Epub 2018 Sep 25. Fluorescent Protein Genes & Plasmids Commonly used fluorescent protein genes and vectors Updated January 25th, 2021 These combined DNA sequence and map files can be opened with SnapGene or the free SnapGene Viewer. GFP/RFP/YFP G418 resistant plasmids - Help please (reply: 1) Destroying GFP fluorescence - (reply: 5) Reduction in EGFP after sorting - (reply: 4) Has anybody added artificial introns into GFP coding sequence. M. 301-251-6638; Contact Us Plasmid Protocol . It is a fertility factor present in some bacteria that permits . 1002/0471140864. An internal control may be a plasmid that constitutively expresses a reporter protein (e. com/result/fluorescence protein gfp reporter plasmid pmaxgfp/product/Amaxa pVIVO-GFP/LacZ plasmids are specifically designed for in vivo experiments. Reporter gene minicircles. pVITRO1-GFP/LacZ allow the ubiquitous and constitutive co-expression of two reporter genes: GFP (green fluorescent protein) and LacZ (β-galactosidase). ) The promoter region was destroyed. For Research Use Only. (No, the promoter is fine   Creating Precise GFP. Size The recombinant plasmid used in this experiment is pGLO plasmids. Highlights: Expresses CD44CR1 with GFP reporter. The plasmid was delivered to HeLa cells. (b) pTET-GFP10 / 11 플라스미드로부터 N 말단 GFP10 및 C 말단 GFP11  26 Feb 2019 Green Fluorescent Protein (GFP) was first isolated from the jelly fish, Aequorea victoria. Chang Liu's lab contains the insert Green Fluorescent Protein with 1 UAG codon and is published in Integr Biol (Camb). 6. pLL-CMV-GFP-T2A-Blast Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus Reliably label the cells of your choice with copGFP driven by the strong CMV promoter (and with blasticidin selection) - great for tracking cells, HTS assays, and more After transformation, the GFP cassette was transferred from the cloning vector into the IncK plasmid via homologous recombination. Amaxa fluorescence protein gfp reporter plasmid pmaxgfp Fluorescence Protein Gfp Reporter Plasmid Pmaxgfp, supplied by Amaxa, used in various techniques. RDB08652 pRS306-SEC71TMD-GFP Plasmid clone to express SEC71 transmembrane domain tagged with GFP in S. 2009;540:301-19. On the basis of pIP501, a green fluorescent protein (GFP)-tagged monitoring tool was constructed for quantifying plasmid mobilization among Gram-positive bacteria and between Gram-positive Enterococcus faecalis and Gram-negative Escherichia coli. Plasmid pCAG-GFP from Dr. It is reported to be a very rapidly-maturing weak dimer. cerevisiae: RDB08658 316-GFP-SED5-SB The green fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent probes. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm. AcGFP1 fluorescent protein · ZsGreen1 fluorescent protein · GFP & GFPuv  The plasmid was used for transfection of HCT116 colorectal carcinoma cells, while data from non-transfected and pEGFP-N1-transfected cells is also shown. #B8 : pVL-GFP is a plasmid transfer vector for expressing target proteins in-fusion with the C-terminal GFP tag. GFP. pscAAV-GFP Control Plasmid.